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hs578t atcc cat  (ATCC)


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    Structured Review

    ATCC hs578t atcc cat
    Hs578t Atcc Cat, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 2322 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hs578t+atcc+cat/Hs+578T/pm31668947-200-95-96
    Average 97 stars, based on 2322 article reviews
    hs578t atcc cat - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    SYBR Green Assay:

    Article Title: Therapeutic Targeting of CDK12/CDK13 in Triple-Negative Breast Cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-Ser2 CTD Cell Signaling Cat#13499S; RRID: AB_2235415 Phospho-Ser5 CTD Cell Signaling Cat#13523S; RRID: AB_2798246 Phospho-Ser7 CTD Abcam Cat#ab126537 Total CTD Abcam Cat#ab817; RRID: AB_306327 ATM Cell Signaling Cat#2873S; RRID: AB_2062659 RAD51 Santa Cruz Cat#sc-8349; RRID: AB_2253533 g-H2AX (Western Blot – IHQ) Cell Signaling Cat#9718S; RRID: AB_2118009 g-H2AX (IF) Millipore Cat#05-636; RRID: AB_2755003 cPARP Cell Signaling Cat#9541S; RRID: AB_331426 Cleaved Caspase 3 (CC3) Cell Signaling Cat#9661S; RRID: AB_2341188 BRCA1 Cell Signaling Cat#9010S; RRID: AB_2228244 Phospho-Ser15 p53 Cell Signaling Cat#9286S; RRID: AB_331741 Phospho-Ser9 p53 Cell Signaling Cat#9288S; RRID: AB_331470 Total p53 Santa Cruz Cat#sc-126; RRID: AB_628082 KI67 Cell Signaling Cat#9129S; RRID: AB_2687446 IRDye 680RD Goat anti-Mouse IgG (H+L) Li-Cor Cat#926-68070; RRID: AB_10956588 CDK12 Novus Cat#NB100-87011; RRID: AB_1199396 CDK13 Millipore Cat#ABE1860 IRDye 800CW Goat anti-Rabbit IgG (H+L) Li-Cor Cat#926-32211; RRID: AB_621843 Alexa-Fluor 488 Goat anti-Rabbit IgG (H+L) Life Technologies Cat#A-11008; RRID: AB_143165 Alexa-Fluor 594 Donkey anti-Mouse IgG (H+L) Life Technologies Cat#A-21203; RRID: AB_2535789 GAPDH Millipore Cat#CB1001-500UG; RRID: AB_2107426 Bacterial and Virus Strains Stbl3 Chemically Competent E. coli Life Technologies C737303 Biological Samples Patient-derived xenografts (PDX) Baylor College of Medicine (Zhang et al., 2013) N/A Chemical, Peptides, and Recombinant Proteins DMSO Sigma-Aldrich Cat#D2650 Odyssey Blocking Buffer Li-Cor Cat#927-40000 Paraformaldehide (PFA) Electron Microscopy Sciences Cat#15710 Cdk12 recombinant protein Proquinase Cat#1483-1484-1 CTDpS7 peptide LifeTein N/A DMEM Life Technologies Cat#11965118 Fetal Bovine Serum Life Technologies Cat#16140071 Insulin Life Technologies Cat#12585014 Cisplatin Biotang Inc Cat#RYG01 Irinotecan LC Laboratories Cat#I-4122 Doxorubicin LC Laboratories Cat#D-4000 Low-gelling-temperature agarose Sigma-Aldrich Cat#A0701 Proteinase K Millipore Cat# 706634 Propidium Iodide Sigma-Aldrich Cat#P4864 Olaparib LC Laboratories Cat#O-9201-50MG Hydroxypropyl-b-Cyclodextrin (hp-BCD) Sigma-Aldrich Cat#389145-25G (Continued on next page) Cancer Cell 36, 1–14.e1–e7, November 11, 2019 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Crystal Violet Alfa Aesar Cat#22866 qScript cDNA SupeMix QuantaBio Cat#95048-100 Power SYBR Green PCR Master Mix Life Technologies Cat#4367659 Bovine Serum Albumin (BSA) Sigma-Aldrich Cat#A3803-50G Hoechst Thermo Scientific Cat#62249 Lipofectamine3000 Life Technologies Cat#L3000015 SR-4835 This paper N/A Critical Commercial Assays RNeasy Plus Mini Kit Qiagen Cat#74136 ADP-Glo Kinase Assay Promega Cat#V9101 CellTiter-Glo Luminescent Cell Viability Assay Promega Cat#G7571 Caspase-Glo 3/7 Promega Cat#G8091 Deposited Data Bioproject – Gene Expression Data BioProject PRJNA530774, https://www.ncbi. nlm.nih.gov/bioproject/528296 Experimental Models: Cell Lines Human: MDA-MB-231 ATCC Cat#HTB-26; RRID: CVCL_0062 Human: MDA-MB-436 ATCC Cat#HTB-130; RRID: CVCL_0623 Human: HS578T ATCC Cat#HTB-126; RRID: CVCL_0332 Human: MDA-MB-468 ATCC Cat#HTB-132; RRID: CVCL_0419 Human: FHC ATCC Cat#CRL-1831; RRID: CVCL_3688 Experimental Models: Organisms/Strains Mouse: Fox Chase SCID Beige Female Charles River Cat#250; RRID: IMSR_CRL:250 Oligonucleotides ATM_F: GCTGACAATCATCACCAAGT This paper N/A ATM_R: GGTTCTCAGCACTATGGGACA This paper N/A ATR_F: CGCTGAACTGTACGTGGAAA This paper N/A ATR_R: CAATTAGTGCCTGGTGAACATC This paper N/A BRCA1_F: CTGCTCAGGGCTATCCTCTCA This paper N/A BRCA1_R: GCTTCTAGTTCAGCCATTTCCTG This paper N/A CD44_F: CGGACACCATGGACAAGTTT This paper N/A CD44_R: GAAAGCCTTGCAGAGGTCAG This paper N/A CDK12_F: CCAATCTGGAACTGGCTCAG This paper N/A CDK12_R: CAAGTGCTGCAGAAGGAATG This paper N/A CDK13_F: GGTGTTTGAATATATGGACC This paper N/A CDK13_R: CAAGTCCAAAGTCTGCAAGTT This paper N/A CCNK_F: CTCCCAAAGAAGAGAACAAAGCA This paper N/A CCNK_R: AGGCAACGGTGGATGAGTG This paper N/A E2F1_F: CATCAGTACCTGGCCGAGAG (Rocca et al., 2019) N/A E2F1_R: CCCGGGGATTTCACACCTTT (Rocca et al., 2019) N/A FANCD2_F: CCCAGAACTGATCAACTCTCCT This paper N/A FANCD2_R: CCATCATCACACGGAAGAAA This paper N/A FANCI_F: CACCACACTTACAGCCCTTG This paper N/A FANCI_R: ATTCCTCCGGAGCTCTGAC This paper N/A GAPDH_F: TCACCAGGGCTGCTTTTAAC This paper N/A GAPDH_R: ATCTCGCTCCTGGAAGATGG This paper N/A KRAS_F: CCTGCTGTGTCGAGAATATCCA (Nakayama et al., 2017) N/A KRAS_R: TTGACGATACAGCTAATTCAGAATCA (Nakayama et al., 2017) N/A NRAS_F: GCGAAGGCTTCCTCTGTGTA (Lau et al., 2017) N/A (Continued on next page) e2 Cancer Cell 36, 1–14.e1–e7, November 11, 2019 ..

    Polymerase Chain Reaction:

    Article Title: Therapeutic Targeting of CDK12/CDK13 in Triple-Negative Breast Cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-Ser2 CTD Cell Signaling Cat#13499S; RRID: AB_2235415 Phospho-Ser5 CTD Cell Signaling Cat#13523S; RRID: AB_2798246 Phospho-Ser7 CTD Abcam Cat#ab126537 Total CTD Abcam Cat#ab817; RRID: AB_306327 ATM Cell Signaling Cat#2873S; RRID: AB_2062659 RAD51 Santa Cruz Cat#sc-8349; RRID: AB_2253533 g-H2AX (Western Blot – IHQ) Cell Signaling Cat#9718S; RRID: AB_2118009 g-H2AX (IF) Millipore Cat#05-636; RRID: AB_2755003 cPARP Cell Signaling Cat#9541S; RRID: AB_331426 Cleaved Caspase 3 (CC3) Cell Signaling Cat#9661S; RRID: AB_2341188 BRCA1 Cell Signaling Cat#9010S; RRID: AB_2228244 Phospho-Ser15 p53 Cell Signaling Cat#9286S; RRID: AB_331741 Phospho-Ser9 p53 Cell Signaling Cat#9288S; RRID: AB_331470 Total p53 Santa Cruz Cat#sc-126; RRID: AB_628082 KI67 Cell Signaling Cat#9129S; RRID: AB_2687446 IRDye 680RD Goat anti-Mouse IgG (H+L) Li-Cor Cat#926-68070; RRID: AB_10956588 CDK12 Novus Cat#NB100-87011; RRID: AB_1199396 CDK13 Millipore Cat#ABE1860 IRDye 800CW Goat anti-Rabbit IgG (H+L) Li-Cor Cat#926-32211; RRID: AB_621843 Alexa-Fluor 488 Goat anti-Rabbit IgG (H+L) Life Technologies Cat#A-11008; RRID: AB_143165 Alexa-Fluor 594 Donkey anti-Mouse IgG (H+L) Life Technologies Cat#A-21203; RRID: AB_2535789 GAPDH Millipore Cat#CB1001-500UG; RRID: AB_2107426 Bacterial and Virus Strains Stbl3 Chemically Competent E. coli Life Technologies C737303 Biological Samples Patient-derived xenografts (PDX) Baylor College of Medicine (Zhang et al., 2013) N/A Chemical, Peptides, and Recombinant Proteins DMSO Sigma-Aldrich Cat#D2650 Odyssey Blocking Buffer Li-Cor Cat#927-40000 Paraformaldehide (PFA) Electron Microscopy Sciences Cat#15710 Cdk12 recombinant protein Proquinase Cat#1483-1484-1 CTDpS7 peptide LifeTein N/A DMEM Life Technologies Cat#11965118 Fetal Bovine Serum Life Technologies Cat#16140071 Insulin Life Technologies Cat#12585014 Cisplatin Biotang Inc Cat#RYG01 Irinotecan LC Laboratories Cat#I-4122 Doxorubicin LC Laboratories Cat#D-4000 Low-gelling-temperature agarose Sigma-Aldrich Cat#A0701 Proteinase K Millipore Cat# 706634 Propidium Iodide Sigma-Aldrich Cat#P4864 Olaparib LC Laboratories Cat#O-9201-50MG Hydroxypropyl-b-Cyclodextrin (hp-BCD) Sigma-Aldrich Cat#389145-25G (Continued on next page) Cancer Cell 36, 1–14.e1–e7, November 11, 2019 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Crystal Violet Alfa Aesar Cat#22866 qScript cDNA SupeMix QuantaBio Cat#95048-100 Power SYBR Green PCR Master Mix Life Technologies Cat#4367659 Bovine Serum Albumin (BSA) Sigma-Aldrich Cat#A3803-50G Hoechst Thermo Scientific Cat#62249 Lipofectamine3000 Life Technologies Cat#L3000015 SR-4835 This paper N/A Critical Commercial Assays RNeasy Plus Mini Kit Qiagen Cat#74136 ADP-Glo Kinase Assay Promega Cat#V9101 CellTiter-Glo Luminescent Cell Viability Assay Promega Cat#G7571 Caspase-Glo 3/7 Promega Cat#G8091 Deposited Data Bioproject – Gene Expression Data BioProject PRJNA530774, https://www.ncbi. nlm.nih.gov/bioproject/528296 Experimental Models: Cell Lines Human: MDA-MB-231 ATCC Cat#HTB-26; RRID: CVCL_0062 Human: MDA-MB-436 ATCC Cat#HTB-130; RRID: CVCL_0623 Human: HS578T ATCC Cat#HTB-126; RRID: CVCL_0332 Human: MDA-MB-468 ATCC Cat#HTB-132; RRID: CVCL_0419 Human: FHC ATCC Cat#CRL-1831; RRID: CVCL_3688 Experimental Models: Organisms/Strains Mouse: Fox Chase SCID Beige Female Charles River Cat#250; RRID: IMSR_CRL:250 Oligonucleotides ATM_F: GCTGACAATCATCACCAAGT This paper N/A ATM_R: GGTTCTCAGCACTATGGGACA This paper N/A ATR_F: CGCTGAACTGTACGTGGAAA This paper N/A ATR_R: CAATTAGTGCCTGGTGAACATC This paper N/A BRCA1_F: CTGCTCAGGGCTATCCTCTCA This paper N/A BRCA1_R: GCTTCTAGTTCAGCCATTTCCTG This paper N/A CD44_F: CGGACACCATGGACAAGTTT This paper N/A CD44_R: GAAAGCCTTGCAGAGGTCAG This paper N/A CDK12_F: CCAATCTGGAACTGGCTCAG This paper N/A CDK12_R: CAAGTGCTGCAGAAGGAATG This paper N/A CDK13_F: GGTGTTTGAATATATGGACC This paper N/A CDK13_R: CAAGTCCAAAGTCTGCAAGTT This paper N/A CCNK_F: CTCCCAAAGAAGAGAACAAAGCA This paper N/A CCNK_R: AGGCAACGGTGGATGAGTG This paper N/A E2F1_F: CATCAGTACCTGGCCGAGAG (Rocca et al., 2019) N/A E2F1_R: CCCGGGGATTTCACACCTTT (Rocca et al., 2019) N/A FANCD2_F: CCCAGAACTGATCAACTCTCCT This paper N/A FANCD2_R: CCATCATCACACGGAAGAAA This paper N/A FANCI_F: CACCACACTTACAGCCCTTG This paper N/A FANCI_R: ATTCCTCCGGAGCTCTGAC This paper N/A GAPDH_F: TCACCAGGGCTGCTTTTAAC This paper N/A GAPDH_R: ATCTCGCTCCTGGAAGATGG This paper N/A KRAS_F: CCTGCTGTGTCGAGAATATCCA (Nakayama et al., 2017) N/A KRAS_R: TTGACGATACAGCTAATTCAGAATCA (Nakayama et al., 2017) N/A NRAS_F: GCGAAGGCTTCCTCTGTGTA (Lau et al., 2017) N/A (Continued on next page) e2 Cancer Cell 36, 1–14.e1–e7, November 11, 2019 ..

    Kinase Assay:

    Article Title: Therapeutic Targeting of CDK12/CDK13 in Triple-Negative Breast Cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-Ser2 CTD Cell Signaling Cat#13499S; RRID: AB_2235415 Phospho-Ser5 CTD Cell Signaling Cat#13523S; RRID: AB_2798246 Phospho-Ser7 CTD Abcam Cat#ab126537 Total CTD Abcam Cat#ab817; RRID: AB_306327 ATM Cell Signaling Cat#2873S; RRID: AB_2062659 RAD51 Santa Cruz Cat#sc-8349; RRID: AB_2253533 g-H2AX (Western Blot – IHQ) Cell Signaling Cat#9718S; RRID: AB_2118009 g-H2AX (IF) Millipore Cat#05-636; RRID: AB_2755003 cPARP Cell Signaling Cat#9541S; RRID: AB_331426 Cleaved Caspase 3 (CC3) Cell Signaling Cat#9661S; RRID: AB_2341188 BRCA1 Cell Signaling Cat#9010S; RRID: AB_2228244 Phospho-Ser15 p53 Cell Signaling Cat#9286S; RRID: AB_331741 Phospho-Ser9 p53 Cell Signaling Cat#9288S; RRID: AB_331470 Total p53 Santa Cruz Cat#sc-126; RRID: AB_628082 KI67 Cell Signaling Cat#9129S; RRID: AB_2687446 IRDye 680RD Goat anti-Mouse IgG (H+L) Li-Cor Cat#926-68070; RRID: AB_10956588 CDK12 Novus Cat#NB100-87011; RRID: AB_1199396 CDK13 Millipore Cat#ABE1860 IRDye 800CW Goat anti-Rabbit IgG (H+L) Li-Cor Cat#926-32211; RRID: AB_621843 Alexa-Fluor 488 Goat anti-Rabbit IgG (H+L) Life Technologies Cat#A-11008; RRID: AB_143165 Alexa-Fluor 594 Donkey anti-Mouse IgG (H+L) Life Technologies Cat#A-21203; RRID: AB_2535789 GAPDH Millipore Cat#CB1001-500UG; RRID: AB_2107426 Bacterial and Virus Strains Stbl3 Chemically Competent E. coli Life Technologies C737303 Biological Samples Patient-derived xenografts (PDX) Baylor College of Medicine (Zhang et al., 2013) N/A Chemical, Peptides, and Recombinant Proteins DMSO Sigma-Aldrich Cat#D2650 Odyssey Blocking Buffer Li-Cor Cat#927-40000 Paraformaldehide (PFA) Electron Microscopy Sciences Cat#15710 Cdk12 recombinant protein Proquinase Cat#1483-1484-1 CTDpS7 peptide LifeTein N/A DMEM Life Technologies Cat#11965118 Fetal Bovine Serum Life Technologies Cat#16140071 Insulin Life Technologies Cat#12585014 Cisplatin Biotang Inc Cat#RYG01 Irinotecan LC Laboratories Cat#I-4122 Doxorubicin LC Laboratories Cat#D-4000 Low-gelling-temperature agarose Sigma-Aldrich Cat#A0701 Proteinase K Millipore Cat# 706634 Propidium Iodide Sigma-Aldrich Cat#P4864 Olaparib LC Laboratories Cat#O-9201-50MG Hydroxypropyl-b-Cyclodextrin (hp-BCD) Sigma-Aldrich Cat#389145-25G (Continued on next page) Cancer Cell 36, 1–14.e1–e7, November 11, 2019 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Crystal Violet Alfa Aesar Cat#22866 qScript cDNA SupeMix QuantaBio Cat#95048-100 Power SYBR Green PCR Master Mix Life Technologies Cat#4367659 Bovine Serum Albumin (BSA) Sigma-Aldrich Cat#A3803-50G Hoechst Thermo Scientific Cat#62249 Lipofectamine3000 Life Technologies Cat#L3000015 SR-4835 This paper N/A Critical Commercial Assays RNeasy Plus Mini Kit Qiagen Cat#74136 ADP-Glo Kinase Assay Promega Cat#V9101 CellTiter-Glo Luminescent Cell Viability Assay Promega Cat#G7571 Caspase-Glo 3/7 Promega Cat#G8091 Deposited Data Bioproject – Gene Expression Data BioProject PRJNA530774, https://www.ncbi. nlm.nih.gov/bioproject/528296 Experimental Models: Cell Lines Human: MDA-MB-231 ATCC Cat#HTB-26; RRID: CVCL_0062 Human: MDA-MB-436 ATCC Cat#HTB-130; RRID: CVCL_0623 Human: HS578T ATCC Cat#HTB-126; RRID: CVCL_0332 Human: MDA-MB-468 ATCC Cat#HTB-132; RRID: CVCL_0419 Human: FHC ATCC Cat#CRL-1831; RRID: CVCL_3688 Experimental Models: Organisms/Strains Mouse: Fox Chase SCID Beige Female Charles River Cat#250; RRID: IMSR_CRL:250 Oligonucleotides ATM_F: GCTGACAATCATCACCAAGT This paper N/A ATM_R: GGTTCTCAGCACTATGGGACA This paper N/A ATR_F: CGCTGAACTGTACGTGGAAA This paper N/A ATR_R: CAATTAGTGCCTGGTGAACATC This paper N/A BRCA1_F: CTGCTCAGGGCTATCCTCTCA This paper N/A BRCA1_R: GCTTCTAGTTCAGCCATTTCCTG This paper N/A CD44_F: CGGACACCATGGACAAGTTT This paper N/A CD44_R: GAAAGCCTTGCAGAGGTCAG This paper N/A CDK12_F: CCAATCTGGAACTGGCTCAG This paper N/A CDK12_R: CAAGTGCTGCAGAAGGAATG This paper N/A CDK13_F: GGTGTTTGAATATATGGACC This paper N/A CDK13_R: CAAGTCCAAAGTCTGCAAGTT This paper N/A CCNK_F: CTCCCAAAGAAGAGAACAAAGCA This paper N/A CCNK_R: AGGCAACGGTGGATGAGTG This paper N/A E2F1_F: CATCAGTACCTGGCCGAGAG (Rocca et al., 2019) N/A E2F1_R: CCCGGGGATTTCACACCTTT (Rocca et al., 2019) N/A FANCD2_F: CCCAGAACTGATCAACTCTCCT This paper N/A FANCD2_R: CCATCATCACACGGAAGAAA This paper N/A FANCI_F: CACCACACTTACAGCCCTTG This paper N/A FANCI_R: ATTCCTCCGGAGCTCTGAC This paper N/A GAPDH_F: TCACCAGGGCTGCTTTTAAC This paper N/A GAPDH_R: ATCTCGCTCCTGGAAGATGG This paper N/A KRAS_F: CCTGCTGTGTCGAGAATATCCA (Nakayama et al., 2017) N/A KRAS_R: TTGACGATACAGCTAATTCAGAATCA (Nakayama et al., 2017) N/A NRAS_F: GCGAAGGCTTCCTCTGTGTA (Lau et al., 2017) N/A (Continued on next page) e2 Cancer Cell 36, 1–14.e1–e7, November 11, 2019 ..

    Cell Viability Assay:

    Article Title: Therapeutic Targeting of CDK12/CDK13 in Triple-Negative Breast Cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-Ser2 CTD Cell Signaling Cat#13499S; RRID: AB_2235415 Phospho-Ser5 CTD Cell Signaling Cat#13523S; RRID: AB_2798246 Phospho-Ser7 CTD Abcam Cat#ab126537 Total CTD Abcam Cat#ab817; RRID: AB_306327 ATM Cell Signaling Cat#2873S; RRID: AB_2062659 RAD51 Santa Cruz Cat#sc-8349; RRID: AB_2253533 g-H2AX (Western Blot – IHQ) Cell Signaling Cat#9718S; RRID: AB_2118009 g-H2AX (IF) Millipore Cat#05-636; RRID: AB_2755003 cPARP Cell Signaling Cat#9541S; RRID: AB_331426 Cleaved Caspase 3 (CC3) Cell Signaling Cat#9661S; RRID: AB_2341188 BRCA1 Cell Signaling Cat#9010S; RRID: AB_2228244 Phospho-Ser15 p53 Cell Signaling Cat#9286S; RRID: AB_331741 Phospho-Ser9 p53 Cell Signaling Cat#9288S; RRID: AB_331470 Total p53 Santa Cruz Cat#sc-126; RRID: AB_628082 KI67 Cell Signaling Cat#9129S; RRID: AB_2687446 IRDye 680RD Goat anti-Mouse IgG (H+L) Li-Cor Cat#926-68070; RRID: AB_10956588 CDK12 Novus Cat#NB100-87011; RRID: AB_1199396 CDK13 Millipore Cat#ABE1860 IRDye 800CW Goat anti-Rabbit IgG (H+L) Li-Cor Cat#926-32211; RRID: AB_621843 Alexa-Fluor 488 Goat anti-Rabbit IgG (H+L) Life Technologies Cat#A-11008; RRID: AB_143165 Alexa-Fluor 594 Donkey anti-Mouse IgG (H+L) Life Technologies Cat#A-21203; RRID: AB_2535789 GAPDH Millipore Cat#CB1001-500UG; RRID: AB_2107426 Bacterial and Virus Strains Stbl3 Chemically Competent E. coli Life Technologies C737303 Biological Samples Patient-derived xenografts (PDX) Baylor College of Medicine (Zhang et al., 2013) N/A Chemical, Peptides, and Recombinant Proteins DMSO Sigma-Aldrich Cat#D2650 Odyssey Blocking Buffer Li-Cor Cat#927-40000 Paraformaldehide (PFA) Electron Microscopy Sciences Cat#15710 Cdk12 recombinant protein Proquinase Cat#1483-1484-1 CTDpS7 peptide LifeTein N/A DMEM Life Technologies Cat#11965118 Fetal Bovine Serum Life Technologies Cat#16140071 Insulin Life Technologies Cat#12585014 Cisplatin Biotang Inc Cat#RYG01 Irinotecan LC Laboratories Cat#I-4122 Doxorubicin LC Laboratories Cat#D-4000 Low-gelling-temperature agarose Sigma-Aldrich Cat#A0701 Proteinase K Millipore Cat# 706634 Propidium Iodide Sigma-Aldrich Cat#P4864 Olaparib LC Laboratories Cat#O-9201-50MG Hydroxypropyl-b-Cyclodextrin (hp-BCD) Sigma-Aldrich Cat#389145-25G (Continued on next page) Cancer Cell 36, 1–14.e1–e7, November 11, 2019 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Crystal Violet Alfa Aesar Cat#22866 qScript cDNA SupeMix QuantaBio Cat#95048-100 Power SYBR Green PCR Master Mix Life Technologies Cat#4367659 Bovine Serum Albumin (BSA) Sigma-Aldrich Cat#A3803-50G Hoechst Thermo Scientific Cat#62249 Lipofectamine3000 Life Technologies Cat#L3000015 SR-4835 This paper N/A Critical Commercial Assays RNeasy Plus Mini Kit Qiagen Cat#74136 ADP-Glo Kinase Assay Promega Cat#V9101 CellTiter-Glo Luminescent Cell Viability Assay Promega Cat#G7571 Caspase-Glo 3/7 Promega Cat#G8091 Deposited Data Bioproject – Gene Expression Data BioProject PRJNA530774, https://www.ncbi. nlm.nih.gov/bioproject/528296 Experimental Models: Cell Lines Human: MDA-MB-231 ATCC Cat#HTB-26; RRID: CVCL_0062 Human: MDA-MB-436 ATCC Cat#HTB-130; RRID: CVCL_0623 Human: HS578T ATCC Cat#HTB-126; RRID: CVCL_0332 Human: MDA-MB-468 ATCC Cat#HTB-132; RRID: CVCL_0419 Human: FHC ATCC Cat#CRL-1831; RRID: CVCL_3688 Experimental Models: Organisms/Strains Mouse: Fox Chase SCID Beige Female Charles River Cat#250; RRID: IMSR_CRL:250 Oligonucleotides ATM_F: GCTGACAATCATCACCAAGT This paper N/A ATM_R: GGTTCTCAGCACTATGGGACA This paper N/A ATR_F: CGCTGAACTGTACGTGGAAA This paper N/A ATR_R: CAATTAGTGCCTGGTGAACATC This paper N/A BRCA1_F: CTGCTCAGGGCTATCCTCTCA This paper N/A BRCA1_R: GCTTCTAGTTCAGCCATTTCCTG This paper N/A CD44_F: CGGACACCATGGACAAGTTT This paper N/A CD44_R: GAAAGCCTTGCAGAGGTCAG This paper N/A CDK12_F: CCAATCTGGAACTGGCTCAG This paper N/A CDK12_R: CAAGTGCTGCAGAAGGAATG This paper N/A CDK13_F: GGTGTTTGAATATATGGACC This paper N/A CDK13_R: CAAGTCCAAAGTCTGCAAGTT This paper N/A CCNK_F: CTCCCAAAGAAGAGAACAAAGCA This paper N/A CCNK_R: AGGCAACGGTGGATGAGTG This paper N/A E2F1_F: CATCAGTACCTGGCCGAGAG (Rocca et al., 2019) N/A E2F1_R: CCCGGGGATTTCACACCTTT (Rocca et al., 2019) N/A FANCD2_F: CCCAGAACTGATCAACTCTCCT This paper N/A FANCD2_R: CCATCATCACACGGAAGAAA This paper N/A FANCI_F: CACCACACTTACAGCCCTTG This paper N/A FANCI_R: ATTCCTCCGGAGCTCTGAC This paper N/A GAPDH_F: TCACCAGGGCTGCTTTTAAC This paper N/A GAPDH_R: ATCTCGCTCCTGGAAGATGG This paper N/A KRAS_F: CCTGCTGTGTCGAGAATATCCA (Nakayama et al., 2017) N/A KRAS_R: TTGACGATACAGCTAATTCAGAATCA (Nakayama et al., 2017) N/A NRAS_F: GCGAAGGCTTCCTCTGTGTA (Lau et al., 2017) N/A (Continued on next page) e2 Cancer Cell 36, 1–14.e1–e7, November 11, 2019 ..

    Gene Expression:

    Article Title: Therapeutic Targeting of CDK12/CDK13 in Triple-Negative Breast Cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-Ser2 CTD Cell Signaling Cat#13499S; RRID: AB_2235415 Phospho-Ser5 CTD Cell Signaling Cat#13523S; RRID: AB_2798246 Phospho-Ser7 CTD Abcam Cat#ab126537 Total CTD Abcam Cat#ab817; RRID: AB_306327 ATM Cell Signaling Cat#2873S; RRID: AB_2062659 RAD51 Santa Cruz Cat#sc-8349; RRID: AB_2253533 g-H2AX (Western Blot – IHQ) Cell Signaling Cat#9718S; RRID: AB_2118009 g-H2AX (IF) Millipore Cat#05-636; RRID: AB_2755003 cPARP Cell Signaling Cat#9541S; RRID: AB_331426 Cleaved Caspase 3 (CC3) Cell Signaling Cat#9661S; RRID: AB_2341188 BRCA1 Cell Signaling Cat#9010S; RRID: AB_2228244 Phospho-Ser15 p53 Cell Signaling Cat#9286S; RRID: AB_331741 Phospho-Ser9 p53 Cell Signaling Cat#9288S; RRID: AB_331470 Total p53 Santa Cruz Cat#sc-126; RRID: AB_628082 KI67 Cell Signaling Cat#9129S; RRID: AB_2687446 IRDye 680RD Goat anti-Mouse IgG (H+L) Li-Cor Cat#926-68070; RRID: AB_10956588 CDK12 Novus Cat#NB100-87011; RRID: AB_1199396 CDK13 Millipore Cat#ABE1860 IRDye 800CW Goat anti-Rabbit IgG (H+L) Li-Cor Cat#926-32211; RRID: AB_621843 Alexa-Fluor 488 Goat anti-Rabbit IgG (H+L) Life Technologies Cat#A-11008; RRID: AB_143165 Alexa-Fluor 594 Donkey anti-Mouse IgG (H+L) Life Technologies Cat#A-21203; RRID: AB_2535789 GAPDH Millipore Cat#CB1001-500UG; RRID: AB_2107426 Bacterial and Virus Strains Stbl3 Chemically Competent E. coli Life Technologies C737303 Biological Samples Patient-derived xenografts (PDX) Baylor College of Medicine (Zhang et al., 2013) N/A Chemical, Peptides, and Recombinant Proteins DMSO Sigma-Aldrich Cat#D2650 Odyssey Blocking Buffer Li-Cor Cat#927-40000 Paraformaldehide (PFA) Electron Microscopy Sciences Cat#15710 Cdk12 recombinant protein Proquinase Cat#1483-1484-1 CTDpS7 peptide LifeTein N/A DMEM Life Technologies Cat#11965118 Fetal Bovine Serum Life Technologies Cat#16140071 Insulin Life Technologies Cat#12585014 Cisplatin Biotang Inc Cat#RYG01 Irinotecan LC Laboratories Cat#I-4122 Doxorubicin LC Laboratories Cat#D-4000 Low-gelling-temperature agarose Sigma-Aldrich Cat#A0701 Proteinase K Millipore Cat# 706634 Propidium Iodide Sigma-Aldrich Cat#P4864 Olaparib LC Laboratories Cat#O-9201-50MG Hydroxypropyl-b-Cyclodextrin (hp-BCD) Sigma-Aldrich Cat#389145-25G (Continued on next page) Cancer Cell 36, 1–14.e1–e7, November 11, 2019 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Crystal Violet Alfa Aesar Cat#22866 qScript cDNA SupeMix QuantaBio Cat#95048-100 Power SYBR Green PCR Master Mix Life Technologies Cat#4367659 Bovine Serum Albumin (BSA) Sigma-Aldrich Cat#A3803-50G Hoechst Thermo Scientific Cat#62249 Lipofectamine3000 Life Technologies Cat#L3000015 SR-4835 This paper N/A Critical Commercial Assays RNeasy Plus Mini Kit Qiagen Cat#74136 ADP-Glo Kinase Assay Promega Cat#V9101 CellTiter-Glo Luminescent Cell Viability Assay Promega Cat#G7571 Caspase-Glo 3/7 Promega Cat#G8091 Deposited Data Bioproject – Gene Expression Data BioProject PRJNA530774, https://www.ncbi. nlm.nih.gov/bioproject/528296 Experimental Models: Cell Lines Human: MDA-MB-231 ATCC Cat#HTB-26; RRID: CVCL_0062 Human: MDA-MB-436 ATCC Cat#HTB-130; RRID: CVCL_0623 Human: HS578T ATCC Cat#HTB-126; RRID: CVCL_0332 Human: MDA-MB-468 ATCC Cat#HTB-132; RRID: CVCL_0419 Human: FHC ATCC Cat#CRL-1831; RRID: CVCL_3688 Experimental Models: Organisms/Strains Mouse: Fox Chase SCID Beige Female Charles River Cat#250; RRID: IMSR_CRL:250 Oligonucleotides ATM_F: GCTGACAATCATCACCAAGT This paper N/A ATM_R: GGTTCTCAGCACTATGGGACA This paper N/A ATR_F: CGCTGAACTGTACGTGGAAA This paper N/A ATR_R: CAATTAGTGCCTGGTGAACATC This paper N/A BRCA1_F: CTGCTCAGGGCTATCCTCTCA This paper N/A BRCA1_R: GCTTCTAGTTCAGCCATTTCCTG This paper N/A CD44_F: CGGACACCATGGACAAGTTT This paper N/A CD44_R: GAAAGCCTTGCAGAGGTCAG This paper N/A CDK12_F: CCAATCTGGAACTGGCTCAG This paper N/A CDK12_R: CAAGTGCTGCAGAAGGAATG This paper N/A CDK13_F: GGTGTTTGAATATATGGACC This paper N/A CDK13_R: CAAGTCCAAAGTCTGCAAGTT This paper N/A CCNK_F: CTCCCAAAGAAGAGAACAAAGCA This paper N/A CCNK_R: AGGCAACGGTGGATGAGTG This paper N/A E2F1_F: CATCAGTACCTGGCCGAGAG (Rocca et al., 2019) N/A E2F1_R: CCCGGGGATTTCACACCTTT (Rocca et al., 2019) N/A FANCD2_F: CCCAGAACTGATCAACTCTCCT This paper N/A FANCD2_R: CCATCATCACACGGAAGAAA This paper N/A FANCI_F: CACCACACTTACAGCCCTTG This paper N/A FANCI_R: ATTCCTCCGGAGCTCTGAC This paper N/A GAPDH_F: TCACCAGGGCTGCTTTTAAC This paper N/A GAPDH_R: ATCTCGCTCCTGGAAGATGG This paper N/A KRAS_F: CCTGCTGTGTCGAGAATATCCA (Nakayama et al., 2017) N/A KRAS_R: TTGACGATACAGCTAATTCAGAATCA (Nakayama et al., 2017) N/A NRAS_F: GCGAAGGCTTCCTCTGTGTA (Lau et al., 2017) N/A (Continued on next page) e2 Cancer Cell 36, 1–14.e1–e7, November 11, 2019 ..

    Multiple Displacement Amplification:

    Article Title: Therapeutic Targeting of CDK12/CDK13 in Triple-Negative Breast Cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-Ser2 CTD Cell Signaling Cat#13499S; RRID: AB_2235415 Phospho-Ser5 CTD Cell Signaling Cat#13523S; RRID: AB_2798246 Phospho-Ser7 CTD Abcam Cat#ab126537 Total CTD Abcam Cat#ab817; RRID: AB_306327 ATM Cell Signaling Cat#2873S; RRID: AB_2062659 RAD51 Santa Cruz Cat#sc-8349; RRID: AB_2253533 g-H2AX (Western Blot – IHQ) Cell Signaling Cat#9718S; RRID: AB_2118009 g-H2AX (IF) Millipore Cat#05-636; RRID: AB_2755003 cPARP Cell Signaling Cat#9541S; RRID: AB_331426 Cleaved Caspase 3 (CC3) Cell Signaling Cat#9661S; RRID: AB_2341188 BRCA1 Cell Signaling Cat#9010S; RRID: AB_2228244 Phospho-Ser15 p53 Cell Signaling Cat#9286S; RRID: AB_331741 Phospho-Ser9 p53 Cell Signaling Cat#9288S; RRID: AB_331470 Total p53 Santa Cruz Cat#sc-126; RRID: AB_628082 KI67 Cell Signaling Cat#9129S; RRID: AB_2687446 IRDye 680RD Goat anti-Mouse IgG (H+L) Li-Cor Cat#926-68070; RRID: AB_10956588 CDK12 Novus Cat#NB100-87011; RRID: AB_1199396 CDK13 Millipore Cat#ABE1860 IRDye 800CW Goat anti-Rabbit IgG (H+L) Li-Cor Cat#926-32211; RRID: AB_621843 Alexa-Fluor 488 Goat anti-Rabbit IgG (H+L) Life Technologies Cat#A-11008; RRID: AB_143165 Alexa-Fluor 594 Donkey anti-Mouse IgG (H+L) Life Technologies Cat#A-21203; RRID: AB_2535789 GAPDH Millipore Cat#CB1001-500UG; RRID: AB_2107426 Bacterial and Virus Strains Stbl3 Chemically Competent E. coli Life Technologies C737303 Biological Samples Patient-derived xenografts (PDX) Baylor College of Medicine (Zhang et al., 2013) N/A Chemical, Peptides, and Recombinant Proteins DMSO Sigma-Aldrich Cat#D2650 Odyssey Blocking Buffer Li-Cor Cat#927-40000 Paraformaldehide (PFA) Electron Microscopy Sciences Cat#15710 Cdk12 recombinant protein Proquinase Cat#1483-1484-1 CTDpS7 peptide LifeTein N/A DMEM Life Technologies Cat#11965118 Fetal Bovine Serum Life Technologies Cat#16140071 Insulin Life Technologies Cat#12585014 Cisplatin Biotang Inc Cat#RYG01 Irinotecan LC Laboratories Cat#I-4122 Doxorubicin LC Laboratories Cat#D-4000 Low-gelling-temperature agarose Sigma-Aldrich Cat#A0701 Proteinase K Millipore Cat# 706634 Propidium Iodide Sigma-Aldrich Cat#P4864 Olaparib LC Laboratories Cat#O-9201-50MG Hydroxypropyl-b-Cyclodextrin (hp-BCD) Sigma-Aldrich Cat#389145-25G (Continued on next page) Cancer Cell 36, 1–14.e1–e7, November 11, 2019 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Crystal Violet Alfa Aesar Cat#22866 qScript cDNA SupeMix QuantaBio Cat#95048-100 Power SYBR Green PCR Master Mix Life Technologies Cat#4367659 Bovine Serum Albumin (BSA) Sigma-Aldrich Cat#A3803-50G Hoechst Thermo Scientific Cat#62249 Lipofectamine3000 Life Technologies Cat#L3000015 SR-4835 This paper N/A Critical Commercial Assays RNeasy Plus Mini Kit Qiagen Cat#74136 ADP-Glo Kinase Assay Promega Cat#V9101 CellTiter-Glo Luminescent Cell Viability Assay Promega Cat#G7571 Caspase-Glo 3/7 Promega Cat#G8091 Deposited Data Bioproject – Gene Expression Data BioProject PRJNA530774, https://www.ncbi. nlm.nih.gov/bioproject/528296 Experimental Models: Cell Lines Human: MDA-MB-231 ATCC Cat#HTB-26; RRID: CVCL_0062 Human: MDA-MB-436 ATCC Cat#HTB-130; RRID: CVCL_0623 Human: HS578T ATCC Cat#HTB-126; RRID: CVCL_0332 Human: MDA-MB-468 ATCC Cat#HTB-132; RRID: CVCL_0419 Human: FHC ATCC Cat#CRL-1831; RRID: CVCL_3688 Experimental Models: Organisms/Strains Mouse: Fox Chase SCID Beige Female Charles River Cat#250; RRID: IMSR_CRL:250 Oligonucleotides ATM_F: GCTGACAATCATCACCAAGT This paper N/A ATM_R: GGTTCTCAGCACTATGGGACA This paper N/A ATR_F: CGCTGAACTGTACGTGGAAA This paper N/A ATR_R: CAATTAGTGCCTGGTGAACATC This paper N/A BRCA1_F: CTGCTCAGGGCTATCCTCTCA This paper N/A BRCA1_R: GCTTCTAGTTCAGCCATTTCCTG This paper N/A CD44_F: CGGACACCATGGACAAGTTT This paper N/A CD44_R: GAAAGCCTTGCAGAGGTCAG This paper N/A CDK12_F: CCAATCTGGAACTGGCTCAG This paper N/A CDK12_R: CAAGTGCTGCAGAAGGAATG This paper N/A CDK13_F: GGTGTTTGAATATATGGACC This paper N/A CDK13_R: CAAGTCCAAAGTCTGCAAGTT This paper N/A CCNK_F: CTCCCAAAGAAGAGAACAAAGCA This paper N/A CCNK_R: AGGCAACGGTGGATGAGTG This paper N/A E2F1_F: CATCAGTACCTGGCCGAGAG (Rocca et al., 2019) N/A E2F1_R: CCCGGGGATTTCACACCTTT (Rocca et al., 2019) N/A FANCD2_F: CCCAGAACTGATCAACTCTCCT This paper N/A FANCD2_R: CCATCATCACACGGAAGAAA This paper N/A FANCI_F: CACCACACTTACAGCCCTTG This paper N/A FANCI_R: ATTCCTCCGGAGCTCTGAC This paper N/A GAPDH_F: TCACCAGGGCTGCTTTTAAC This paper N/A GAPDH_R: ATCTCGCTCCTGGAAGATGG This paper N/A KRAS_F: CCTGCTGTGTCGAGAATATCCA (Nakayama et al., 2017) N/A KRAS_R: TTGACGATACAGCTAATTCAGAATCA (Nakayama et al., 2017) N/A NRAS_F: GCGAAGGCTTCCTCTGTGTA (Lau et al., 2017) N/A (Continued on next page) e2 Cancer Cell 36, 1–14.e1–e7, November 11, 2019 ..



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